Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1290443..1291061 Replicon chromosome
Accession NZ_CP028758
Organism Escherichia coli strain A16

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY69_RS05985 Protein ID WP_001291435.1
Coordinates 1290443..1290661 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY69_RS05990 Protein ID WP_000344800.1
Coordinates 1290687..1291061 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY69_RS05950 (1285732) 1285732..1286304 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY69_RS05955 (1286335) 1286335..1286646 - 312 WP_000409911.1 MGMT family protein -
AOY69_RS05965 (1287025) 1287025..1287378 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY69_RS05970 (1287420) 1287420..1288970 - 1551 WP_119457962.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY69_RS05975 (1289134) 1289134..1289604 - 471 WP_000136192.1 YlaC family protein -
AOY69_RS05980 (1289720) 1289720..1290271 - 552 WP_000102564.1 maltose O-acetyltransferase -
AOY69_RS05985 (1290443) 1290443..1290661 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY69_RS05990 (1290687) 1290687..1291061 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY69_RS05995 (1291607) 1291607..1294756 - 3150 WP_201479756.1 efflux RND transporter permease AcrB -
AOY69_RS06000 (1294779) 1294779..1295972 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103737 WP_001291435.1 NZ_CP028758:c1290661-1290443 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103737 NZ_CP028758:c1290661-1290443 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103737 WP_000344800.1 NZ_CP028758:c1291061-1290687 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103737 NZ_CP028758:c1291061-1290687 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References