Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 807707..808257 Replicon chromosome
Accession NZ_CP028758
Organism Escherichia coli strain A16

Toxin (Protein)


Gene name ccdB Uniprot ID A0A788AHB0
Locus tag AOY69_RS03770 Protein ID WP_028132515.1
Coordinates 807943..808257 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AOY69_RS03765 Protein ID WP_000125566.1
Coordinates 807707..807940 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY69_RS03740 (802778) 802778..803065 + 288 WP_000203741.1 ferredoxin-like protein FixX -
AOY69_RS03745 (803123) 803123..804454 + 1332 WP_023307570.1 MFS transporter -
AOY69_RS03750 (804565) 804565..805095 + 531 WP_023307571.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AOY69_RS03755 (805088) 805088..806950 + 1863 WP_000377186.1 glutathione-regulated potassium-efflux system protein KefC -
AOY69_RS03760 (807142) 807142..807621 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AOY69_RS03765 (807707) 807707..807940 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY69_RS03770 (807943) 807943..808257 + 315 WP_028132515.1 CcdB family protein Toxin
AOY69_RS03775 (808254) 808254..809102 - 849 WP_000257185.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AOY69_RS03780 (809109) 809109..809486 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AOY69_RS03785 (809489) 809489..810310 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AOY69_RS03790 (810307) 810307..811296 - 990 WP_033814582.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AOY69_RS03795 (811296) 811296..812582 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11694.82 Da        Isoelectric Point: 9.0855

>T103735 WP_028132515.1 NZ_CP028758:807943-808257 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRILPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T103735 NZ_CP028758:807943-808257 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCTCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT103735 WP_000125566.1 NZ_CP028758:807707-807940 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT103735 NZ_CP028758:807707-807940 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A788AHB0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References