Detailed information of TA system TA103734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 684527..685103
• Replicon: chromosome
• Accession: NZ_CP028758
• Organism: Escherichia coli strain A16

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A061KXE4
• Locus tag: AOY69_RS03155
• Protein ID: WP_001295743.1
• Coordinates: 684527..684814 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: AOY69_RS03160
• Protein ID: WP_119457955.1
• Coordinates: 684801..685103 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY69_RS03140 (680112)	680112..681491	-	1380	WP_033814748.1	restriction endonuclease subunit S	-
AOY69_RS03145 (681481)	681481..683100	-	1620	WP_033814747.1	class I SAM-dependent DNA methyltransferase	-
AOY69_RS03150 (683164)	683164..684330	-	1167	WP_047623658.1	restriction endonuclease	-
AOY69_RS03155 (684527)	684527..684814	+	288	WP_001295743.1	BrnT family toxin	Toxin
AOY69_RS03160 (684801)	684801..685103	+	303	WP_119457955.1	BrnA antitoxin family protein	Antitoxin
AOY69_RS03165 (685137)	685137..686093	-	957	WP_001297640.1	GTPase	-
AOY69_RS03170 (686104)	686104..686307	-	204	WP_000467859.1	YbdD/YjiX family protein	-
AOY69_RS03175 (686357)	686357..688507	-	2151	WP_001309197.1	pyruvate/proton symporter BtsT	-
AOY69_RS03180 (688900)	688900..689412	-	513	WP_001175455.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11156.62 Da
• Isoelectric Point: 7.4697

>T103734 WP_001295743.1 NZ_CP028758:684527-684814 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T103734 NZ_CP028758:684527-684814 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCT
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11512.04 Da
• Isoelectric Point: 9.4646

>AT103734 WP_119457955.1 NZ_CP028758:684801-685103 [Escherichia coli]
MSMVKHKRGAPPTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT103734 NZ_CP028758:684801-685103 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGGCACCCCCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A061KXE4