Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 467493..467990 Replicon chromosome
Accession NZ_CP028758
Organism Escherichia coli strain A16

Toxin (Protein)


Gene name ghoT Uniprot ID A0A163EQM5
Locus tag AOY69_RS02115 Protein ID WP_001672275.1
Coordinates 467817..467990 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AOY69_RS02110 Protein ID WP_000398619.1
Coordinates 467493..467789 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY69_RS02095 (462853) 462853..463668 + 816 WP_230047538.1 S6 family peptidase -
AOY69_RS02100 (463656) 463656..466781 + 3126 WP_230047537.1 autotransporter outer membrane beta-barrel domain-containing protein -
AOY69_RS02105 (466994) 466994..467266 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY69_RS02110 (467493) 467493..467789 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY69_RS02115 (467817) 467817..467990 + 174 WP_001672275.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY69_RS02120 (468109) 468109..469626 - 1518 WP_001295074.1 lysine--tRNA ligase -
AOY69_RS02125 (469863) 469863..471320 - 1458 WP_119457808.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espC 451774..476844 25070


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6569.09 Da        Isoelectric Point: 10.2288

>T103732 WP_001672275.1 NZ_CP028758:467817-467990 [Escherichia coli]
MALFSKILIFYVIGVNISFIIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103732 NZ_CP028758:467817-467990 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTATCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT103732 WP_000398619.1 NZ_CP028758:467493-467789 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103732 NZ_CP028758:467493-467789 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A163EQM5

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References