Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4488440..4489026 Replicon chromosome
Accession NZ_CP028757
Organism Escherichia coli strain A17

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag AOY70_RS21735 Protein ID WP_042014162.1
Coordinates 4488796..4489026 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY70_RS21730 Protein ID WP_000593555.1
Coordinates 4488440..4488799 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY70_RS21705 (4484572) 4484572..4485516 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
AOY70_RS21710 (4485513) 4485513..4486346 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AOY70_RS21715 (4486346) 4486346..4487110 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
AOY70_RS21720 (4487107) 4487107..4487913 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
AOY70_RS21725 (4487919) 4487919..4488320 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOY70_RS21730 (4488440) 4488440..4488799 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY70_RS21735 (4488796) 4488796..4489026 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY70_RS21740 (4489130) 4489130..4490254 - 1125 WP_001216257.1 ABC transporter permease -
AOY70_RS21745 (4490254) 4490254..4492989 - 2736 WP_028132003.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T103731 WP_042014162.1 NZ_CP028757:c4489026-4488796 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T103731 NZ_CP028757:c4489026-4488796 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103731 WP_000593555.1 NZ_CP028757:c4488799-4488440 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103731 NZ_CP028757:c4488799-4488440 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References