Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3453160..3453785 Replicon chromosome
Accession NZ_CP028757
Organism Escherichia coli strain A17

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag AOY70_RS16655 Protein ID WP_000911329.1
Coordinates 3453160..3453558 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag AOY70_RS16660 Protein ID WP_000450524.1
Coordinates 3453558..3453785 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY70_RS16635 (3449038) 3449038..3449238 + 201 WP_000383836.1 YpfN family protein -
AOY70_RS16640 (3449348) 3449348..3450046 - 699 WP_000679823.1 esterase -
AOY70_RS16645 (3450120) 3450120..3452135 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
AOY70_RS16650 (3452150) 3452150..3453013 - 864 WP_001267498.1 neutral zinc metallopeptidase -
AOY70_RS16655 (3453160) 3453160..3453558 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
AOY70_RS16660 (3453558) 3453558..3453785 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
AOY70_RS16665 (3453940) 3453940..3454653 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
AOY70_RS16670 (3454866) 3454866..3455900 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
AOY70_RS16675 (3455917) 3455917..3456795 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
AOY70_RS16680 (3456941) 3456941..3457513 + 573 WP_000176191.1 glycine cleavage system transcriptional repressor -
AOY70_RS16685 (3457513) 3457513..3457983 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T103727 WP_000911329.1 NZ_CP028757:c3453558-3453160 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T103727 NZ_CP028757:c3453558-3453160 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT103727 WP_000450524.1 NZ_CP028757:c3453785-3453558 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT103727 NZ_CP028757:c3453785-3453558 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References