Detailed information of TA system TA103716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2385451..2386089
• Replicon: chromosome
• Accession: NZ_CP028757
• Organism: Escherichia coli strain A17

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY70_RS11560
• Protein ID: WP_000813794.1
• Coordinates: 2385451..2385627 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY70_RS11565
• Protein ID: WP_001270285.1
• Coordinates: 2385673..2386089 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY70_RS11540 (2381070)	2381070..2382284	-	1215	WP_071601296.1	BenE family transporter YdcO	-
AOY70_RS11545 (2382337)	2382337..2382873	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AOY70_RS11550 (2382946)	2382946..2384907	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY70_RS11555 (2384999)	2384999..2385229	-	231	WP_000494244.1	YncJ family protein	-
AOY70_RS11560 (2385451)	2385451..2385627	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY70_RS11565 (2385673)	2385673..2386089	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY70_RS11570 (2386168)	2386168..2387574	+	1407	WP_000760594.1	PLP-dependent aminotransferase family protein	-
AOY70_RS11575 (2387819)	2387819..2388964	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
AOY70_RS11580 (2388982)	2388982..2389995	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
AOY70_RS11585 (2389996)	2389996..2390937	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2379882..2381030	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T103716 WP_000813794.1 NZ_CP028757:2385451-2385627 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103716 NZ_CP028757:2385451-2385627 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT103716 WP_001270285.1 NZ_CP028757:2385673-2386089 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103716 NZ_CP028757:2385673-2386089 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB