Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1238241..1238859 Replicon chromosome
Accession NZ_CP028757
Organism Escherichia coli strain A17

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY70_RS05795 Protein ID WP_001291435.1
Coordinates 1238241..1238459 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY70_RS05800 Protein ID WP_000344800.1
Coordinates 1238485..1238859 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY70_RS05760 (1233531) 1233531..1234103 + 573 WP_000779826.1 YbaY family lipoprotein -
AOY70_RS05765 (1234134) 1234134..1234445 - 312 WP_000409911.1 MGMT family protein -
AOY70_RS05775 (1234824) 1234824..1235177 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY70_RS05780 (1235219) 1235219..1236769 - 1551 WP_069915398.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY70_RS05785 (1236933) 1236933..1237403 - 471 WP_000136192.1 YlaC family protein -
AOY70_RS05790 (1237519) 1237519..1238070 - 552 WP_000102564.1 maltose O-acetyltransferase -
AOY70_RS05795 (1238241) 1238241..1238459 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY70_RS05800 (1238485) 1238485..1238859 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY70_RS05805 (1239405) 1239405..1242554 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY70_RS05810 (1242577) 1242577..1243770 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103711 WP_001291435.1 NZ_CP028757:c1238459-1238241 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103711 NZ_CP028757:c1238459-1238241 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103711 WP_000344800.1 NZ_CP028757:c1238859-1238485 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103711 NZ_CP028757:c1238859-1238485 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References