Detailed information of TA system TA103710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1203944..1204781
• Replicon: chromosome
• Accession: NZ_CP028757
• Organism: Escherichia coli strain A17

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY70_RS05625
• Protein ID: WP_000227784.1
• Coordinates: 1203944..1204486 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY70_RS05630
• Protein ID: WP_001297137.1
• Coordinates: 1204470..1204781 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY70_RS05605 (1199483)	1199483..1200394	-	912	WP_000705849.1	2-dehydropantoate 2-reductase	-
AOY70_RS05610 (1200562)	1200562..1201053	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY70_RS05615 (1201181)	1201181..1202545	-	1365	WP_001000978.1	MFS transporter	-
AOY70_RS05620 (1202953)	1202953..1203888	+	936	WP_001365761.1	tetratricopeptide repeat protein	-
AOY70_RS05625 (1203944)	1203944..1204486	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY70_RS05630 (1204470)	1204470..1204781	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY70_RS05635 (1204966)	1204966..1205856	-	891	WP_000971336.1	heme o synthase	-
AOY70_RS05640 (1205868)	1205868..1206197	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY70_RS05645 (1206197)	1206197..1206811	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY70_RS05650 (1206801)	1206801..1208792	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY70_RS05655 (1208814)	1208814..1209761	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103710 WP_000227784.1 NZ_CP028757:c1204486-1203944 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103710 NZ_CP028757:c1204486-1203944 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103710 WP_001297137.1 NZ_CP028757:c1204781-1204470 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103710 NZ_CP028757:c1204781-1204470 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3