Detailed information of TA system TA103704

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4565054..4565886
• Replicon: chromosome
• Accession: NZ_CP028756
• Organism: Escherichia coli strain A19

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: AOY72_RS22170
• Protein ID: WP_000854753.1
• Coordinates: 4565512..4565886 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AOY72_RS22165
• Protein ID: WP_001383228.1
• Coordinates: 4565054..4565422 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY72_RS22135 (4561885)	4561885..4562340	+	456	WP_000581504.1	IrmA family protein	-
AOY72_RS22140 (4562419)	4562419..4562652	+	234	WP_001119729.1	DUF905 family protein	-
AOY72_RS22145 (4562752)	4562752..4563570	+	819	WP_137584764.1	DUF932 domain-containing protein	-
AOY72_RS22150 (4563625)	4563625..4564110	+	486	WP_201479231.1	antirestriction protein	-
AOY72_RS22155 (4564125)	4564125..4564601	+	477	WP_073461968.1	RadC family protein	-
AOY72_RS22160 (4564670)	4564670..4564891	+	222	WP_000692323.1	DUF987 domain-containing protein	-
AOY72_RS22165 (4565054)	4565054..4565422	+	369	WP_001383228.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY72_RS22170 (4565512)	4565512..4565886	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
AOY72_RS22175 (4565937)	4565937..4566371	+	435	WP_001556361.1	DUF5983 family protein	-
AOY72_RS22180 (4566383)	4566383..4566580	+	198	WP_000839281.1	DUF957 domain-containing protein	-
AOY72_RS22185 (4566677)	4566677..4567246	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
AOY72_RS22190 (4567995)	4567995..4569533	+	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T103704 WP_000854753.1 NZ_CP028756:4565512-4565886 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T103704 NZ_CP028756:4565512-4565886 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13617.32 Da
• Isoelectric Point: 6.2058

>AT103704 WP_001383228.1 NZ_CP028756:4565054-4565422 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT103704 NZ_CP028756:4565054-4565422 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
ACCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0