Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3668408..3669026 Replicon chromosome
Accession NZ_CP028756
Organism Escherichia coli strain A19

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY72_RS17825 Protein ID WP_001291435.1
Coordinates 3668808..3669026 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY72_RS17820 Protein ID WP_000344800.1
Coordinates 3668408..3668782 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY72_RS17810 (3663497) 3663497..3664690 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY72_RS17815 (3664713) 3664713..3667862 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY72_RS17820 (3668408) 3668408..3668782 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY72_RS17825 (3668808) 3668808..3669026 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY72_RS17830 (3669197) 3669197..3669748 + 552 WP_000102578.1 maltose O-acetyltransferase -
AOY72_RS17835 (3669864) 3669864..3670334 + 471 WP_000136192.1 YlaC family protein -
AOY72_RS17840 (3670498) 3670498..3672048 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY72_RS17845 (3672090) 3672090..3672443 - 354 WP_000878141.1 DUF1428 family protein -
AOY72_RS17855 (3672822) 3672822..3673133 + 312 WP_000409911.1 MGMT family protein -
AOY72_RS17860 (3673164) 3673164..3673736 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103700 WP_001291435.1 NZ_CP028756:3668808-3669026 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103700 NZ_CP028756:3668808-3669026 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103700 WP_000344800.1 NZ_CP028756:3668408-3668782 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103700 NZ_CP028756:3668408-3668782 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References