Detailed information of TA system TA103689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1416094..1416719
• Replicon: chromosome
• Accession: NZ_CP028756
• Organism: Escherichia coli strain A19

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOY72_RS06810
• Protein ID: WP_000911330.1
• Coordinates: 1416321..1416719 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AOY72_RS06805
• Protein ID: WP_000450524.1
• Coordinates: 1416094..1416321 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY72_RS06780 (1411897)	1411897..1412367	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
AOY72_RS06785 (1412367)	1412367..1412939	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AOY72_RS06790 (1413085)	1413085..1413963	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AOY72_RS06795 (1413980)	1413980..1415014	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
AOY72_RS06800 (1415227)	1415227..1415940	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AOY72_RS06805 (1416094)	1416094..1416321	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AOY72_RS06810 (1416321)	1416321..1416719	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOY72_RS06815 (1416866)	1416866..1417729	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
AOY72_RS06820 (1417744)	1417744..1419759	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
AOY72_RS06825 (1419833)	1419833..1420531	+	699	WP_000679823.1	esterase	-
AOY72_RS06830 (1420641)	1420641..1420841	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T103689 WP_000911330.1 NZ_CP028756:1416321-1416719 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T103689 NZ_CP028756:1416321-1416719 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT103689 WP_000450524.1 NZ_CP028756:1416094-1416321 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT103689 NZ_CP028756:1416094-1416321 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33