Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4451373..4451870 Replicon chromosome
Accession NZ_CP028755
Organism Escherichia coli strain A20

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY73_RS21610 Protein ID WP_001173343.1
Coordinates 4451373..4451546 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag AOY73_RS21615 Protein ID WP_001312331.1
Coordinates 4451574..4451870 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY73_RS21600 (4448043) 4448043..4449500 + 1458 WP_000856849.1 dipeptide/tripeptide permease DtpC -
AOY73_RS21605 (4449737) 4449737..4451254 + 1518 WP_000003807.1 lysine--tRNA ligase -
AOY73_RS21610 (4451373) 4451373..4451546 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY73_RS21615 (4451574) 4451574..4451870 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY73_RS21620 (4452099) 4452099..4452371 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY73_RS21625 (4452383) 4452383..4452613 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
AOY73_RS21630 (4452794) 4452794..4454425 + 1632 WP_001216466.1 sensor histidine kinase -
AOY73_RS21635 (4454422) 4454422..4455141 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103681 WP_001173343.1 NZ_CP028755:c4451546-4451373 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103681 NZ_CP028755:c4451546-4451373 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT103681 WP_001312331.1 NZ_CP028755:c4451870-4451574 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103681 NZ_CP028755:c4451870-4451574 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References