Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4119631..4120181 Replicon chromosome
Accession NZ_CP028755
Organism Escherichia coli strain A20

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag AOY73_RS20065 Protein ID WP_001160966.1
Coordinates 4119631..4119945 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AOY73_RS20070 Protein ID WP_000125566.1
Coordinates 4119948..4120181 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY73_RS20040 (4115306) 4115306..4116592 + 1287 WP_096979946.1 peptidylprolyl isomerase SurA -
AOY73_RS20045 (4116592) 4116592..4117581 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AOY73_RS20050 (4117578) 4117578..4118399 + 822 WP_001065366.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AOY73_RS20055 (4118402) 4118402..4118779 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AOY73_RS20060 (4118786) 4118786..4119634 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AOY73_RS20065 (4119631) 4119631..4119945 - 315 WP_001160966.1 CcdB family protein Toxin
AOY73_RS20070 (4119948) 4119948..4120181 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY73_RS20075 (4120267) 4120267..4120746 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
AOY73_RS20080 (4120938) 4120938..4122800 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
AOY73_RS20085 (4122793) 4122793..4123323 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AOY73_RS20090 (4123431) 4123431..4124762 - 1332 WP_021534632.1 MFS transporter -
AOY73_RS20095 (4124821) 4124821..4125108 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T103680 WP_001160966.1 NZ_CP028755:c4119945-4119631 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T103680 NZ_CP028755:c4119945-4119631 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT103680 WP_000125566.1 NZ_CP028755:c4120181-4119948 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT103680 NZ_CP028755:c4120181-4119948 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References