Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1047851..1048505 Replicon chromosome
Accession NZ_CP028755
Organism Escherichia coli strain A20

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY73_RS05145 Protein ID WP_000244781.1
Coordinates 1048098..1048505 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY73_RS05140 Protein ID WP_000354046.1
Coordinates 1047851..1048117 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY73_RS05120 (1043939) 1043939..1045372 - 1434 WP_024262092.1 6-phospho-beta-glucosidase BglA -
AOY73_RS05125 (1045417) 1045417..1045728 + 312 WP_001182964.1 N(4)-acetylcytidine aminohydrolase -
AOY73_RS05130 (1045892) 1045892..1046551 + 660 WP_000250274.1 hemolysin III family protein -
AOY73_RS05135 (1046628) 1046628..1047608 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
AOY73_RS05140 (1047851) 1047851..1048117 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY73_RS05145 (1048098) 1048098..1048505 + 408 WP_000244781.1 protein YgfX Toxin
AOY73_RS05150 (1048545) 1048545..1049066 - 522 WP_001055874.1 flavodoxin FldB -
AOY73_RS05155 (1049178) 1049178..1050074 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY73_RS05160 (1050099) 1050099..1050809 + 711 WP_000715226.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY73_RS05165 (1050815) 1050815..1052548 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103663 WP_000244781.1 NZ_CP028755:1048098-1048505 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103663 NZ_CP028755:1048098-1048505 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103663 WP_000354046.1 NZ_CP028755:1047851-1048117 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103663 NZ_CP028755:1047851-1048117 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTGATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References