Detailed information of TA system TA103662

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 918735..919533
• Replicon: chromosome
• Accession: NZ_CP028755
• Organism: Escherichia coli strain A20

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AOY73_RS04455
• Protein ID: WP_096980599.1
• Coordinates: 918735..919112 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L4NTN5
• Locus tag: AOY73_RS04460
• Protein ID: WP_001605875.1
• Coordinates: 919159..919533 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY73_RS04420 (913817)	913817..914422	+	606	WP_001350472.1	type II secretion system minor pseudopilin GspJ	-
AOY73_RS04425 (914419)	914419..915396	+	978	WP_000633183.1	type II secretion system minor pseudopilin GspK	-
AOY73_RS04430 (915393)	915393..916571	+	1179	WP_000097216.1	type II secretion system protein GspL	-
AOY73_RS04435 (916573)	916573..917109	+	537	WP_000942821.1	GspM family type II secretion system protein YghD	-
AOY73_RS04440 (917389)	917389..918231	-	843	WP_240755127.1	DUF4942 domain-containing protein	-
AOY73_RS04445 (918316)	918316..918513	-	198	WP_023563519.1	DUF957 domain-containing protein	-
AOY73_RS04450 (918589)	918589..918738	-	150	Protein_876	DUF5983 family protein	-
AOY73_RS04455 (918735)	918735..919112	-	378	WP_096980599.1	TA system toxin CbtA family protein	Toxin
AOY73_RS04460 (919159)	919159..919533	-	375	WP_001605875.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
AOY73_RS04465 (919609)	919609..919830	-	222	WP_000692323.1	DUF987 domain-containing protein	-
AOY73_RS04470 (919899)	919899..920375	-	477	WP_001186181.1	RadC family protein	-
AOY73_RS04475 (920391)	920391..920876	-	486	WP_000213727.1	antirestriction protein	-
AOY73_RS04480 (920968)	920968..921786	-	819	WP_001234393.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14119.12 Da
• Isoelectric Point: 7.3249

>T103662 WP_096980599.1 NZ_CP028755:c919112-918735 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWHTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFIVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T103662 NZ_CP028755:c919112-918735 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCATACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTATCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13594.42 Da
• Isoelectric Point: 5.4554

>AT103662 WP_001605875.1 NZ_CP028755:c919533-919159 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT103662 NZ_CP028755:c919533-919159 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGGCCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L4NTN5