Detailed information of TA system TA103656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3639889..3640472
• Replicon: chromosome
• Accession: NZ_CP028754
• Organism: Escherichia coli strain A21

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AOY74_RS17565
• Protein ID: WP_022296743.1
• Coordinates: 3639889..3640224 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AOY74_RS17570
• Protein ID: WP_022296742.1
• Coordinates: 3640224..3640472 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY74_RS17550 (3635775)	3635775..3637073	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
AOY74_RS17555 (3637161)	3637161..3638798	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
AOY74_RS17560 (3639026)	3639026..3639817	-	792	WP_001071631.1	nucleoside triphosphate pyrophosphohydrolase	-
AOY74_RS17565 (3639889)	3639889..3640224	-	336	WP_022296743.1	endoribonuclease MazF	Toxin
AOY74_RS17570 (3640224)	3640224..3640472	-	249	WP_022296742.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AOY74_RS17575 (3640550)	3640550..3642784	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
AOY74_RS17580 (3642832)	3642832..3644133	-	1302	WP_000046819.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12127.99 Da
• Isoelectric Point: 8.4777

>T103656 WP_022296743.1 NZ_CP028754:c3640224-3639889 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLYVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T103656 NZ_CP028754:c3640224-3639889 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTATGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9297.70 Da
• Isoelectric Point: 4.5837

>AT103656 WP_022296742.1 NZ_CP028754:c3640472-3640224 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGAPKDKE
VW

Nucleotide

• Length: 249 bp

>AT103656 NZ_CP028754:c3640472-3640224 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGCGCCGAAAGATAAGGAA
GTCTGGTAA