Detailed information of TA system TA103645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2368664..2370252
• Replicon: chromosome
• Accession: NZ_CP028754
• Organism: Escherichia coli strain A21

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: AOY74_RS11420
• Protein ID: WP_201482007.1
• Coordinates: 2368664..2369986 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A061YNW4
• Locus tag: AOY74_RS11425
• Protein ID: WP_001296076.1
• Coordinates: 2369986..2370252 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY74_RS11410 (2366130)	2366130..2366840	-	711	WP_001195177.1	fimbria/pilus periplasmic chaperone	-
AOY74_RS11415 (2367202)	2367202..2367765	-	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
AOY74_RS11420 (2368664)	2368664..2369986	-	1323	WP_201482007.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
AOY74_RS11425 (2369986)	2369986..2370252	-	267	WP_001296076.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
AOY74_RS11430 (2370560)	2370560..2371318	+	759	WP_001524715.1	trans-aconitate 2-methyltransferase	-
AOY74_RS11435 (2371322)	2371322..2372236	-	915	WP_001385356.1	bestrophin family protein	-
AOY74_RS11440 (2372433)	2372433..2373884	-	1452	WP_000854638.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49298.94 Da
• Isoelectric Point: 7.4464

>T103645 WP_201482007.1 NZ_CP028754:c2369986-2368664 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLVPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYLLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Nucleotide

• Length: 1323 bp

>T103645 NZ_CP028754:c2369986-2368664 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGTGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAGTCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCTGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9987.34 Da
• Isoelectric Point: 4.9632

>AT103645 WP_001296076.1 NZ_CP028754:c2370252-2369986 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT103645 NZ_CP028754:c2370252-2369986 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A061YNW4