Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1317241..1317859 Replicon chromosome
Accession NZ_CP028754
Organism Escherichia coli strain A21

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY74_RS06185 Protein ID WP_001291435.1
Coordinates 1317241..1317459 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY74_RS06190 Protein ID WP_000344800.1
Coordinates 1317485..1317859 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY74_RS06150 (1312529) 1312529..1313101 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY74_RS06155 (1313132) 1313132..1313443 - 312 WP_000409908.1 MGMT family protein -
AOY74_RS06165 (1313822) 1313822..1314175 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY74_RS06170 (1314217) 1314217..1315767 - 1551 WP_022296625.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY74_RS06175 (1315931) 1315931..1316401 - 471 WP_000136192.1 YlaC family protein -
AOY74_RS06180 (1316517) 1316517..1317068 - 552 WP_000102539.1 maltose O-acetyltransferase -
AOY74_RS06185 (1317241) 1317241..1317459 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY74_RS06190 (1317485) 1317485..1317859 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY74_RS06195 (1318404) 1318404..1321553 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
AOY74_RS06200 (1321576) 1321576..1322769 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103634 WP_001291435.1 NZ_CP028754:c1317459-1317241 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103634 NZ_CP028754:c1317459-1317241 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103634 WP_000344800.1 NZ_CP028754:c1317859-1317485 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103634 NZ_CP028754:c1317859-1317485 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References