Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 843430..843980 Replicon chromosome
Accession NZ_CP028754
Organism Escherichia coli strain A21

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag AOY74_RS03900 Protein ID WP_001160966.1
Coordinates 843666..843980 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AOY74_RS03895 Protein ID WP_000125566.1
Coordinates 843430..843663 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY74_RS03870 (838503) 838503..838790 + 288 WP_000203741.1 ferredoxin-like protein FixX -
AOY74_RS03875 (838849) 838849..840180 + 1332 WP_022296925.1 MFS transporter -
AOY74_RS03880 (840288) 840288..840818 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AOY74_RS03885 (840811) 840811..842673 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
AOY74_RS03890 (842865) 842865..843344 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AOY74_RS03895 (843430) 843430..843663 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY74_RS03900 (843666) 843666..843980 + 315 WP_001160966.1 CcdB family protein Toxin
AOY74_RS03905 (843977) 843977..844825 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AOY74_RS03910 (844832) 844832..845209 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AOY74_RS03915 (845212) 845212..846033 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AOY74_RS03920 (846030) 846030..847019 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AOY74_RS03925 (847019) 847019..848305 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T103632 WP_001160966.1 NZ_CP028754:843666-843980 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T103632 NZ_CP028754:843666-843980 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT103632 WP_000125566.1 NZ_CP028754:843430-843663 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT103632 NZ_CP028754:843430-843663 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References