Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1885198..1885788 Replicon chromosome
Accession NZ_CP028753
Organism Escherichia coli strain A22

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag AOY75_RS08865 Protein ID WP_000373724.1
Coordinates 1885456..1885788 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag AOY75_RS08860 Protein ID WP_000288812.1
Coordinates 1885198..1885455 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY75_RS08840 (1882811) 1882811..1883947 + 1137 WP_000420971.1 ISAs1-like element ISEc1 family transposase -
AOY75_RS08845 (1884180) 1884180..1884647 + 468 WP_000201267.1 hypothetical protein -
AOY75_RS08850 (1884644) 1884644..1884850 + 207 WP_000983730.1 helix-turn-helix transcriptional regulator -
AOY75_RS08855 (1884909) 1884909..1885100 - 192 WP_077561361.1 hypothetical protein -
AOY75_RS08860 (1885198) 1885198..1885455 + 258 WP_000288812.1 antitoxin Antitoxin
AOY75_RS08865 (1885456) 1885456..1885788 + 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AOY75_RS08870 (1885835) 1885835..1886257 - 423 WP_001164104.1 hypothetical protein -
AOY75_RS08875 (1886636) 1886636..1887514 + 879 WP_000511773.1 integrase domain-containing protein -
AOY75_RS08880 (1888465) 1888465..1888923 + 459 WP_000526101.1 IS200/IS605-like element IS200C family transposase -
AOY75_RS08885 (1889062) 1889062..1890324 - 1263 WP_001609141.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1856065..1909806 53741
- inside IScluster/Tn - - 1882811..1888923 6112


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T103606 WP_000373724.1 NZ_CP028753:1885456-1885788 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T103606 NZ_CP028753:1885456-1885788 [Escherichia coli]
ATGGACAGAGGGGAAATCTGGCTGGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTTGTTCCCGTTACCAGCGGCGGAAACTTCGCCCGTG
CAGCCGGTTTTACCGTCTCACTGGACGGTGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT103606 WP_000288812.1 NZ_CP028753:1885198-1885455 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT103606 NZ_CP028753:1885198-1885455 [Escherichia coli]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGTTGTTGGCGCAGTGCGATCCGCACGCAGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References