Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 927253..927907 Replicon chromosome
Accession NZ_CP028753
Organism Escherichia coli strain A22

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY75_RS04525 Protein ID WP_000244781.1
Coordinates 927500..927907 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY75_RS04520 Protein ID WP_000354046.1
Coordinates 927253..927519 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY75_RS04500 (923341) 923341..924774 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
AOY75_RS04505 (924819) 924819..925130 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AOY75_RS04510 (925294) 925294..925953 + 660 WP_000250274.1 hemolysin III family protein -
AOY75_RS04515 (926030) 926030..927010 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
AOY75_RS04520 (927253) 927253..927519 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY75_RS04525 (927500) 927500..927907 + 408 WP_000244781.1 protein YgfX Toxin
AOY75_RS04530 (927947) 927947..928468 - 522 WP_001055874.1 flavodoxin FldB -
AOY75_RS04535 (928580) 928580..929476 + 897 WP_000806633.1 site-specific tyrosine recombinase XerD -
AOY75_RS04540 (929501) 929501..930211 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY75_RS04545 (930217) 930217..931950 + 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103603 WP_000244781.1 NZ_CP028753:927500-927907 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103603 NZ_CP028753:927500-927907 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103603 WP_000354046.1 NZ_CP028753:927253-927519 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103603 NZ_CP028753:927253-927519 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References