Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4096186..4096683 Replicon chromosome
Accession NZ_CP028752
Organism Escherichia coli strain A23

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY76_RS20000 Protein ID WP_001173343.1
Coordinates 4096186..4096359 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AOY76_RS20005 Protein ID WP_000398619.1
Coordinates 4096387..4096683 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY76_RS19990 (4092856) 4092856..4094313 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
AOY76_RS19995 (4094550) 4094550..4096067 + 1518 WP_240770825.1 lysine--tRNA ligase -
AOY76_RS20000 (4096186) 4096186..4096359 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY76_RS20005 (4096387) 4096387..4096683 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY76_RS20010 (4096910) 4096910..4097182 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY76_RS20015 (4097194) 4097194..4097424 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
AOY76_RS20020 (4097605) 4097605..4099236 + 1632 WP_001216477.1 sensor histidine kinase -
AOY76_RS20025 (4099233) 4099233..4099952 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103601 WP_001173343.1 NZ_CP028752:c4096359-4096186 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103601 NZ_CP028752:c4096359-4096186 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT103601 WP_000398619.1 NZ_CP028752:c4096683-4096387 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103601 NZ_CP028752:c4096683-4096387 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References