Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3378488..3379106 Replicon chromosome
Accession NZ_CP028752
Organism Escherichia coli strain A23

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY76_RS16505 Protein ID WP_001291435.1
Coordinates 3378888..3379106 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY76_RS16500 Protein ID WP_000344800.1
Coordinates 3378488..3378862 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY76_RS16490 (3373577) 3373577..3374770 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY76_RS16495 (3374793) 3374793..3377942 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY76_RS16500 (3378488) 3378488..3378862 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY76_RS16505 (3378888) 3378888..3379106 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY76_RS16510 (3379278) 3379278..3379829 + 552 WP_000102564.1 maltose O-acetyltransferase -
AOY76_RS16515 (3379945) 3379945..3380415 + 471 WP_000136192.1 YlaC family protein -
AOY76_RS16520 (3380579) 3380579..3382129 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY76_RS16525 (3382171) 3382171..3382524 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY76_RS16535 (3382903) 3382903..3383214 + 312 WP_000409911.1 MGMT family protein -
AOY76_RS16540 (3383245) 3383245..3383817 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103599 WP_001291435.1 NZ_CP028752:3378888-3379106 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103599 NZ_CP028752:3378888-3379106 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103599 WP_000344800.1 NZ_CP028752:3378488-3378862 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103599 NZ_CP028752:3378488-3378862 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References