Detailed information of TA system TA103598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3002676..3003381
• Replicon: chromosome
• Accession: NZ_CP028752
• Organism: Escherichia coli strain A23

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: AOY76_RS14645
• Protein ID: WP_000539521.1
• Coordinates: 3002676..3003062 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AOY76_RS14650
• Protein ID: WP_001280945.1
• Coordinates: 3003052..3003381 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY76_RS14625 (2998680)	2998680..2999306	+	627	WP_001295292.1	glutathione S-transferase GstB	-
AOY76_RS14630 (2999303)	2999303..3000418	-	1116	WP_240770771.1	aldose sugar dehydrogenase YliI	-
AOY76_RS14635 (3000529)	3000529..3000912	-	384	WP_000497137.1	biofilm formation regulator BssR	-
AOY76_RS14640 (3001125)	3001125..3002450	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
AOY76_RS14645 (3002676)	3002676..3003062	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOY76_RS14650 (3003052)	3003052..3003381	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
AOY76_RS14655 (3003451)	3003451..3004779	-	1329	WP_240770772.1	GGDEF domain-containing protein	-
AOY76_RS14660 (3004787)	3004787..3006700	-	1914	Protein_2886	cyclic diguanylate phosphodiesterase	-
AOY76_RS14665 (3006757)	3006757..3007454	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
AOY76_RS14670 (3007471)	3007471..3007911	-	441	Protein_2888	CHASE9 sensor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3006951..3007454	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T103598 WP_000539521.1 NZ_CP028752:3002676-3003062 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T103598 NZ_CP028752:3002676-3003062 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT103598 WP_001280945.1 NZ_CP028752:3003052-3003381 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT103598 NZ_CP028752:3003052-3003381 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6