Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2435479..2436117 Replicon chromosome
Accession NZ_CP028752
Organism Escherichia coli strain A23

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AOY76_RS11830 Protein ID WP_000813794.1
Coordinates 2435941..2436117 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AOY76_RS11825 Protein ID WP_001270286.1
Coordinates 2435479..2435895 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY76_RS11805 (2430629) 2430629..2431570 - 942 WP_001251313.1 ABC transporter permease -
AOY76_RS11810 (2431571) 2431571..2432584 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
AOY76_RS11815 (2432602) 2432602..2433749 - 1148 Protein_2326 ABC transporter substrate-binding protein -
AOY76_RS11820 (2433994) 2433994..2435400 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AOY76_RS11825 (2435479) 2435479..2435895 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AOY76_RS11830 (2435941) 2435941..2436117 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AOY76_RS11835 (2436339) 2436339..2436569 + 231 WP_000494244.1 YncJ family protein -
AOY76_RS11840 (2436661) 2436661..2438622 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AOY76_RS11845 (2438695) 2438695..2439231 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AOY76_RS11850 (2439284) 2439284..2440498 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T103597 WP_000813794.1 NZ_CP028752:c2436117-2435941 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T103597 NZ_CP028752:c2436117-2435941 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT103597 WP_001270286.1 NZ_CP028752:c2435895-2435479 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT103597 NZ_CP028752:c2435895-2435479 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References