Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1514757..1515375 Replicon chromosome
Accession NZ_CP028751
Organism Escherichia coli strain A24

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY77_RS07335 Protein ID WP_001291435.1
Coordinates 1514757..1514975 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY77_RS07340 Protein ID WP_000344800.1
Coordinates 1515001..1515375 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY77_RS07300 (1510044) 1510044..1510616 + 573 WP_000779830.1 YbaY family lipoprotein -
AOY77_RS07305 (1510647) 1510647..1510958 - 312 WP_000409908.1 MGMT family protein -
AOY77_RS07315 (1511337) 1511337..1511690 + 354 WP_000878138.1 DUF1428 family protein -
AOY77_RS07320 (1511732) 1511732..1513282 - 1551 WP_201472075.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY77_RS07325 (1513446) 1513446..1513916 - 471 WP_000136192.1 YlaC family protein -
AOY77_RS07330 (1514032) 1514032..1514583 - 552 WP_105471636.1 maltose O-acetyltransferase -
AOY77_RS07335 (1514757) 1514757..1514975 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY77_RS07340 (1515001) 1515001..1515375 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY77_RS07345 (1515920) 1515920..1519069 - 3150 WP_001132481.1 efflux RND transporter permease AcrB -
AOY77_RS07350 (1519092) 1519092..1520285 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103564 WP_001291435.1 NZ_CP028751:c1514975-1514757 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103564 NZ_CP028751:c1514975-1514757 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103564 WP_000344800.1 NZ_CP028751:c1515375-1515001 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103564 NZ_CP028751:c1515375-1515001 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References