Detailed information of TA system TA103563

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1326086..1326921
• Replicon: chromosome
• Accession: NZ_CP028751
• Organism: Escherichia coli strain A24

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: AOY77_RS06445
• Protein ID: WP_000854700.1
• Coordinates: 1326544..1326921 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AOY77_RS06440
• Protein ID: WP_001531802.1
• Coordinates: 1326086..1326454 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY77_RS06420 (1323752)	1323752..1324570	+	819	WP_001234709.1	DUF932 domain-containing protein	-
AOY77_RS06425 (1324662)	1324662..1325147	+	486	WP_201471955.1	antirestriction protein	-
AOY77_RS06430 (1325163)	1325163..1325639	+	477	WP_001186726.1	RadC family protein	-
AOY77_RS06435 (1325702)	1325702..1325923	+	222	WP_000692329.1	DUF987 domain-containing protein	-
AOY77_RS06440 (1326086)	1326086..1326454	+	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY77_RS06445 (1326544)	1326544..1326921	+	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
AOY77_RS06450 (1326918)	1326918..1327406	+	489	WP_000761726.1	DUF5983 family protein	-
AOY77_RS06455 (1327418)	1327418..1327615	+	198	WP_000839247.1	DUF957 domain-containing protein	-
AOY77_RS06460 (1327700)	1327700..1328554	+	855	WP_024174313.1	DUF4942 domain-containing protein	-
AOY77_RS06465 (1328647)	1328647..1329966	-	1320	WP_000144686.1	site-specific integrase	-
AOY77_RS06470 (1330331)	1330331..1330666	+	336	Protein_1246	Arm DNA-binding domain-containing protein	-
AOY77_RS06475 (1331103)	1331103..1331597	-	495	WP_001059463.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vat / yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1300359..1375625	75266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T103563 WP_000854700.1 NZ_CP028751:1326544-1326921 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T103563 NZ_CP028751:1326544-1326921 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCTGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCGGGATTC
AGCCCCTGTACCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT103563 WP_001531802.1 NZ_CP028751:1326086-1326454 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT103563 NZ_CP028751:1326086-1326454 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454A1I2