Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1014017..1014567 Replicon chromosome
Accession NZ_CP028751
Organism Escherichia coli strain A24

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag AOY77_RS04990 Protein ID WP_001160966.1
Coordinates 1014253..1014567 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag AOY77_RS04985 Protein ID WP_000125568.1
Coordinates 1014017..1014250 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY77_RS04960 (1009090) 1009090..1009377 + 288 WP_000203741.1 ferredoxin-like protein FixX -
AOY77_RS04965 (1009436) 1009436..1010767 + 1332 WP_001183198.1 MFS transporter -
AOY77_RS04970 (1010875) 1010875..1011405 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AOY77_RS04975 (1011398) 1011398..1013260 + 1863 WP_201472205.1 glutathione-regulated potassium-efflux system protein KefC -
AOY77_RS04980 (1013452) 1013452..1013931 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AOY77_RS04985 (1014017) 1014017..1014250 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY77_RS04990 (1014253) 1014253..1014567 + 315 WP_001160966.1 CcdB family protein Toxin
AOY77_RS04995 (1014564) 1014564..1015412 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AOY77_RS05000 (1015419) 1015419..1015796 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AOY77_RS05005 (1015799) 1015799..1016620 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AOY77_RS05010 (1016617) 1016617..1017606 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AOY77_RS05015 (1017606) 1017606..1018892 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T103561 WP_001160966.1 NZ_CP028751:1014253-1014567 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T103561 NZ_CP028751:1014253-1014567 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT103561 WP_000125568.1 NZ_CP028751:1014017-1014250 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT103561 NZ_CP028751:1014017-1014250 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References