Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 859801..860377 Replicon chromosome
Accession NZ_CP028751
Organism Escherichia coli strain A24

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag AOY77_RS04175 Protein ID WP_001350372.1
Coordinates 859801..860088 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag AOY77_RS04180 Protein ID WP_000063155.1
Coordinates 860075..860377 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY77_RS04160 (855434) 855434..856765 - 1332 WP_000505211.1 restriction endonuclease subunit S -
AOY77_RS04165 (856755) 856755..858374 - 1620 WP_001029741.1 class I SAM-dependent DNA methyltransferase -
AOY77_RS04170 (858438) 858438..859604 - 1167 WP_000800823.1 restriction endonuclease -
AOY77_RS04175 (859801) 859801..860088 + 288 WP_001350372.1 BrnT family toxin Toxin
AOY77_RS04180 (860075) 860075..860377 + 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
AOY77_RS04185 (860411) 860411..861367 - 957 WP_001600951.1 GTPase -
AOY77_RS04190 (861378) 861378..861581 - 204 WP_000467859.1 YbdD/YjiX family protein -
AOY77_RS04195 (861699) 861699..863849 - 2151 WP_001298085.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T103558 WP_001350372.1 NZ_CP028751:859801-860088 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T103558 NZ_CP028751:859801-860088 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT103558 WP_000063155.1 NZ_CP028751:860075-860377 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT103558 NZ_CP028751:860075-860377 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References