Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4257413..4258044 Replicon chromosome
Accession NZ_CP028750
Organism Escherichia coli strain A25

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag AOY78_RS20850 Protein ID WP_001260301.1
Coordinates 4257769..4258044 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY78_RS20845 Protein ID WP_000593555.1
Coordinates 4257413..4257772 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY78_RS20820 (4253545) 4253545..4254489 + 945 WP_000947071.1 nickel ABC transporter permease subunit NikB -
AOY78_RS20825 (4254486) 4254486..4255319 + 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
AOY78_RS20830 (4255319) 4255319..4256083 + 765 WP_001136232.1 nickel import ATP-binding protein NikD -
AOY78_RS20835 (4256080) 4256080..4256886 + 807 WP_000173679.1 nickel import ATP-binding protein NikE -
AOY78_RS20840 (4256892) 4256892..4257293 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOY78_RS20845 (4257413) 4257413..4257772 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY78_RS20850 (4257769) 4257769..4258044 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY78_RS20855 (4258117) 4258117..4259241 - 1125 WP_024242098.1 ABC transporter permease -
AOY78_RS20860 (4259241) 4259241..4261976 - 2736 Protein_4067 ribosome-associated ATPase/putative transporter RbbA -
AOY78_RS20865 (4261973) 4261973..4263040 - 1068 WP_024242100.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T103552 WP_001260301.1 NZ_CP028750:c4258044-4257769 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103552 NZ_CP028750:c4258044-4257769 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTGCGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103552 WP_000593555.1 NZ_CP028750:c4257772-4257413 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103552 NZ_CP028750:c4257772-4257413 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References