Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-RelB |
| Location | 4230165..4230760 | Replicon | chromosome |
| Accession | NZ_CP028750 | ||
| Organism | Escherichia coli strain A25 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | A0A826P6U8 |
| Locus tag | AOY78_RS20695 | Protein ID | WP_000155160.1 |
| Coordinates | 4230165..4230542 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | A0A827G8B6 |
| Locus tag | AOY78_RS20700 | Protein ID | WP_000557316.1 |
| Coordinates | 4230539..4230760 (-) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AOY78_RS20675 (4225741) | 4225741..4226811 | - | 1071 | WP_000907827.1 | sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC | - |
| AOY78_RS20680 (4226813) | 4226813..4227658 | - | 846 | WP_000572184.1 | sn-glycerol-3-phosphate ABC transporter permease UgpE | - |
| AOY78_RS20685 (4227655) | 4227655..4228542 | - | 888 | WP_000099282.1 | sn-glycerol-3-phosphate ABC transporter permease UgpA | - |
| AOY78_RS20690 (4228640) | 4228640..4229956 | - | 1317 | WP_000803580.1 | sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB | - |
| AOY78_RS20695 (4230165) | 4230165..4230542 | - | 378 | WP_000155160.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| AOY78_RS20700 (4230539) | 4230539..4230760 | - | 222 | WP_000557316.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| AOY78_RS20705 (4230848) | 4230848..4231561 | - | 714 | WP_000416895.1 | high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF | - |
| AOY78_RS20710 (4231563) | 4231563..4232330 | - | 768 | WP_000082101.1 | high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG | - |
| AOY78_RS20715 (4232327) | 4232327..4233604 | - | 1278 | WP_000803819.1 | branched chain amino acid ABC transporter permease LivM | - |
| AOY78_RS20720 (4233601) | 4233601..4234527 | - | 927 | WP_001295097.1 | high-affinity branched-chain amino acid ABC transporter permease LivH | - |
| AOY78_RS20725 (4234575) | 4234575..4235684 | - | 1110 | WP_001297972.1 | high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 4225001..4233604 | 8603 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13945.20 Da Isoelectric Point: 6.4830
>T103551 WP_000155160.1 NZ_CP028750:c4230542-4230165 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGLILAANPEFVELTVDVAAGKVSLEDIVTRLRQHGT
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGLILAANPEFVELTVDVAAGKVSLEDIVTRLRQHGT
Download Length: 378 bp
>T103551 NZ_CP028750:c4230542-4230165 [Escherichia coli]
ATGACCATTCAGTTTATCTCTGCGGAGGAGATAATTTATTTCCACGATAAGTTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTACGAGTATGAAGGCGTTTCCGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATTTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCCTCATACTTGCTGCAAACCCAGAGTTTGTGGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACACGGAACCTGA
ATGACCATTCAGTTTATCTCTGCGGAGGAGATAATTTATTTCCACGATAAGTTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTACGAGTATGAAGGCGTTTCCGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATTTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCCTCATACTTGCTGCAAACCCAGAGTTTGTGGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACACGGAACCTGA
Antitoxin
Download Length: 74 a.a. Molecular weight: 8106.25 Da Isoelectric Point: 5.5759
>AT103551 WP_000557316.1 NZ_CP028750:c4230760-4230539 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIMELADK
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIMELADK
Download Length: 222 bp
>AT103551 NZ_CP028750:c4230760-4230539 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGACAGAACCTTGCATTGGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCCGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CGGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTATGGAGTTAGCGGATAAATGA
ATGCATACCATGAACTACAGCGAAGCGCGACAGAACCTTGCATTGGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCCGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CGGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTATGGAGTTAGCGGATAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A826P6U8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A827G8B6 |