Detailed information of TA system TA103550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4194585..4195339
• Replicon: chromosome
• Accession: NZ_CP028750
• Organism: Escherichia coli strain A25

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A822TTG1
• Locus tag: AOY78_RS20535
• Protein ID: WP_021560068.1
• Coordinates: 4194854..4195339 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: AOY78_RS20530
• Protein ID: WP_000801915.1
• Coordinates: 4194585..4194863 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY78_RS20525 (4191813)	4191813..4194518	+	2706	WP_000906934.1	HTH-type transcriptional regulator MalT	-
AOY78_RS20530 (4194585)	4194585..4194863	+	279	WP_000801915.1	DUF1778 domain-containing protein	Antitoxin
AOY78_RS20535 (4194854)	4194854..4195339	+	486	WP_021560068.1	GNAT family N-acetyltransferase	Toxin
AOY78_RS20540 (4195389)	4195389..4196417	-	1029	WP_024247668.1	RNA 3'-terminal phosphate cyclase	-
AOY78_RS20545 (4196421)	4196421..4197647	-	1227	WP_001105471.1	RNA-splicing ligase RtcB	-
AOY78_RS20550 (4197866)	4197866..4198480	+	615	Protein_4005	DNA-binding transcriptional regulator RtcR	-
AOY78_RS20555 (4198462)	4198462..4199220	-	759	WP_001296480.1	DeoR/GlpR family transcriptional regulator	-
AOY78_RS20560 (4199237)	4199237..4200067	-	831	WP_000928723.1	rhomboid family intramembrane serine protease GlpG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17680.60 Da
• Isoelectric Point: 9.4066

>T103550 WP_021560068.1 NZ_CP028750:4194854-4195339 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSSLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T103550 NZ_CP028750:4194854-4195339 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCAGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGCTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGGGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10450.97 Da
• Isoelectric Point: 4.6336

>AT103550 WP_000801915.1 NZ_CP028750:4194585-4194863 [Escherichia coli]
MKPESKETPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT103550 NZ_CP028750:4194585-4194863 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAACACCAATCAATATCAGGGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTGGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A822TTG1