Detailed information of TA system TA103532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1899365..1900149
• Replicon: chromosome
• Accession: NZ_CP028750
• Organism: Escherichia coli strain A25

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: AOY78_RS09235
• Protein ID: WP_000613626.1
• Coordinates: 1899365..1899859 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: AOY78_RS09240
• Protein ID: WP_000718446.1
• Coordinates: 1899856..1900149 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY78_RS09215 (1894558)	1894558..1895655	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
AOY78_RS09220 (1895655)	1895655..1896596	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
AOY78_RS09225 (1896662)	1896662..1898305	+	1644	WP_032171110.1	flagellar hook-associated protein FlgK	-
AOY78_RS09230 (1898317)	1898317..1899270	+	954	WP_022645524.1	flagellar hook-associated protein FlgL	-
AOY78_RS09235 (1899365)	1899365..1899859	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
AOY78_RS09240 (1899856)	1899856..1900149	-	294	WP_000718446.1	DUF1778 domain-containing protein	Antitoxin
AOY78_RS09245 (1900283)	1900283..1903456	-	3174	WP_096841489.1	ribonuclease E	-
AOY78_RS09250 (1904029)	1904029..1904988	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T103532 WP_000613626.1 NZ_CP028750:c1899859-1899365 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T103532 NZ_CP028750:c1899859-1899365 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11053.63 Da
• Isoelectric Point: 10.5423

>AT103532 WP_000718446.1 NZ_CP028750:c1900149-1899856 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT103532 NZ_CP028750:c1900149-1899856 [Escherichia coli]
ATGGCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9