103530

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 786060..786592 Replicon chromosome
Accession NZ_CP028750
Organism Escherichia coli strain A25

Toxin (Protein)


Gene name ccdB Uniprot ID A0A2A7M2E8
Locus tag AOY78_RS03750 Protein ID WP_001160959.1
Coordinates 786296..786592 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AOY78_RS03745 Protein ID WP_000125566.1
Coordinates 786060..786293 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY78_RS03720 (781133) 781133..781420 + 288 WP_000203741.1 ferredoxin-like protein FixX -
AOY78_RS03725 (781479) 781479..782810 + 1332 WP_001183198.1 MFS transporter -
AOY78_RS03730 (782918) 782918..783448 + 531 WP_201488375.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AOY78_RS03735 (783441) 783441..785303 + 1863 WP_096891018.1 glutathione-regulated potassium-efflux system protein KefC -
AOY78_RS03740 (785495) 785495..785974 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AOY78_RS03745 (786060) 786060..786293 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY78_RS03750 (786296) 786296..786592 + 297 WP_001160959.1 CcdB family protein Toxin
AOY78_RS03755 (786589) 786589..787437 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AOY78_RS03760 (787444) 787444..787821 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AOY78_RS03765 (787824) 787824..788645 - 822 WP_001065367.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AOY78_RS03770 (788642) 788642..789631 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AOY78_RS03775 (789631) 789631..790917 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-97)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11089.98 Da        Isoelectric Point: 8.0406

>T103530 WP_001160959.1 NZ_CP028750:786296-786592 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPFIRIRPPERLNTILLLVDGKEYVLMTHETETVPVNALGTKFCDA
SAHRTLIKGALDFMLDGI

Download         Length: 297 bp

>T103530 NZ_CP028750:786296-786592 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTTATCCGTATTCGCCCTCCAGAACGGCTTAACACAATCCTTTTACTGGTAG
ACGGCAAAGAATATGTGCTCATGACGCATGAGACTGAAACTGTTCCAGTTAACGCGCTGGGAACGAAATTTTGCGATGCC
AGCGCGCACCGAACGTTGATTAAAGGGGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT103530 WP_000125566.1 NZ_CP028750:786060-786293 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT103530 NZ_CP028750:786060-786293 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A7M2E8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References