Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4263460..4263957 Replicon chromosome
Accession NZ_CP028749
Organism Escherichia coli strain A26

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY79_RS20735 Protein ID WP_001173343.1
Coordinates 4263460..4263633 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag AOY79_RS20740 Protein ID WP_001312331.1
Coordinates 4263661..4263957 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY79_RS20725 (4260130) 4260130..4261587 + 1458 WP_021516688.1 dipeptide/tripeptide permease DtpC -
AOY79_RS20730 (4261824) 4261824..4263341 + 1518 WP_000003806.1 lysine--tRNA ligase -
AOY79_RS20735 (4263460) 4263460..4263633 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY79_RS20740 (4263661) 4263661..4263957 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY79_RS20745 (4264186) 4264186..4264458 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY79_RS20750 (4264470) 4264470..4264700 - 231 WP_021516687.1 4Fe-4S mono-cluster protein YjdI -
AOY79_RS20755 (4264881) 4264881..4266512 + 1632 WP_001216466.1 sensor histidine kinase -
AOY79_RS20760 (4266509) 4266509..4267228 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103524 WP_001173343.1 NZ_CP028749:c4263633-4263460 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103524 NZ_CP028749:c4263633-4263460 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT103524 WP_001312331.1 NZ_CP028749:c4263957-4263661 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103524 NZ_CP028749:c4263957-4263661 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References