Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3423538..3424156 Replicon chromosome
Accession NZ_CP028749
Organism Escherichia coli strain A26

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY79_RS16700 Protein ID WP_001291435.1
Coordinates 3423938..3424156 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY79_RS16695 Protein ID WP_000344800.1
Coordinates 3423538..3423912 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY79_RS16685 (3418628) 3418628..3419821 + 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY79_RS16690 (3419844) 3419844..3422993 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY79_RS16695 (3423538) 3423538..3423912 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY79_RS16700 (3423938) 3423938..3424156 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY79_RS16705 (3424329) 3424329..3424880 + 552 WP_000102539.1 maltose O-acetyltransferase -
AOY79_RS16710 (3424996) 3424996..3425466 + 471 WP_000136192.1 YlaC family protein -
AOY79_RS16715 (3425630) 3425630..3427180 + 1551 WP_089638034.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY79_RS16720 (3427222) 3427222..3427575 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY79_RS16730 (3427954) 3427954..3428265 + 312 WP_000409908.1 MGMT family protein -
AOY79_RS16735 (3428296) 3428296..3428868 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103519 WP_001291435.1 NZ_CP028749:3423938-3424156 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103519 NZ_CP028749:3423938-3424156 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103519 WP_000344800.1 NZ_CP028749:3423538-3423912 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103519 NZ_CP028749:3423538-3423912 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References