Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3856106..3856760 Replicon chromosome
Accession NZ_CP028748
Organism Escherichia coli strain A27

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AOY80_RS18535 Protein ID WP_000244777.1
Coordinates 3856106..3856513 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY80_RS18540 Protein ID WP_000354046.1
Coordinates 3856494..3856760 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY80_RS18515 (3852063) 3852063..3853796 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
AOY80_RS18520 (3853802) 3853802..3854512 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY80_RS18525 (3854537) 3854537..3855433 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY80_RS18530 (3855545) 3855545..3856066 + 522 WP_001055874.1 flavodoxin FldB -
AOY80_RS18535 (3856106) 3856106..3856513 - 408 WP_000244777.1 protein YgfX Toxin
AOY80_RS18540 (3856494) 3856494..3856760 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY80_RS18545 (3857003) 3857003..3857983 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY80_RS18550 (3858179) 3858179..3858838 - 660 WP_000250274.1 hemolysin III family protein -
AOY80_RS18555 (3859002) 3859002..3859313 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
AOY80_RS18560 (3859358) 3859358..3860791 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
AOY80_RS18565 (3860848) 3860848..3861591 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T103501 WP_000244777.1 NZ_CP028748:c3856513-3856106 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103501 NZ_CP028748:c3856513-3856106 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103501 WP_000354046.1 NZ_CP028748:c3856760-3856494 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103501 NZ_CP028748:c3856760-3856494 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References