Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-RelB |
| Location | 2455323..2455849 | Replicon | chromosome |
| Accession | NZ_CP028748 | ||
| Organism | Escherichia coli strain A27 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | AOY80_RS11870 | Protein ID | WP_096933764.1 |
| Coordinates | 2455323..2455610 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | S1F6D3 |
| Locus tag | AOY80_RS11875 | Protein ID | WP_000534858.1 |
| Coordinates | 2455610..2455849 (-) | Length | 80 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AOY80_RS11820 (2450349) | 2450349..2450564 | - | 216 | WP_000839590.1 | phage lysis protein EssD | - |
| AOY80_RS11825 (2450784) | 2450784..2450954 | + | 171 | WP_211180497.1 | putative zinc-binding protein YnfU | - |
| AOY80_RS11830 (2451316) | 2451316..2451531 | - | 216 | WP_000066484.1 | cold shock-like protein CspB | - |
| AOY80_RS11835 (2451832) | 2451832..2452044 | + | 213 | WP_000087756.1 | cold shock-like protein CspF | - |
| AOY80_RS11840 (2452099) | 2452099..2452188 | + | 90 | WP_120795389.1 | hypothetical protein | - |
| AOY80_RS11845 (2452466) | 2452466..2453218 | - | 753 | WP_001047135.1 | antitermination protein | - |
| AOY80_RS11850 (2453232) | 2453232..2454281 | - | 1050 | WP_001265199.1 | DUF968 domain-containing protein | - |
| AOY80_RS11855 (2454283) | 2454283..2454561 | - | 279 | WP_012304870.1 | hypothetical protein | - |
| AOY80_RS11860 (2454628) | 2454628..2454879 | - | 252 | WP_000980994.1 | protein Rem | - |
| AOY80_RS11865 (2455096) | 2455096..2455251 | - | 156 | WP_000813254.1 | type I toxin-antitoxin system toxin HokD | - |
| AOY80_RS11870 (2455323) | 2455323..2455610 | - | 288 | WP_096933764.1 | type II toxin-antitoxin system mRNA interferase RelE | Toxin |
| AOY80_RS11875 (2455610) | 2455610..2455849 | - | 240 | WP_000534858.1 | type II toxin-antitoxin system antitoxin RelB | Antitoxin |
| AOY80_RS11880 (2455874) | 2455874..2456179 | + | 306 | WP_001326990.1 | protein YdfV | - |
| AOY80_RS11885 (2456382) | 2456382..2456714 | + | 333 | WP_001301033.1 | FlxA-like family protein | - |
| AOY80_RS11890 (2457151) | 2457151..2457300 | - | 150 | WP_011443592.1 | protein YdfW | - |
| AOY80_RS11895 (2457373) | 2457373..2458443 | - | 1071 | Protein_2313 | ISNCY family transposase | - |
| AOY80_RS11905 (2460458) | 2460458..2460745 | - | 288 | Protein_2315 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | vgrG/tssI / rhs/PAAR / rhs/PAAR / espR1 | 2092760..2505590 | 412830 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11238.30 Da Isoelectric Point: 10.4221
>T103490 WP_096933764.1 NZ_CP028748:c2455610-2455323 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLKSPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVIFVISVGK
RERSEVYSEAVKRIL
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLKSPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVIFVISVGK
RERSEVYSEAVKRIL
Download Length: 288 bp
>T103490 NZ_CP028748:c2455610-2455323 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTAAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCATTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTAAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCATTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA
Antitoxin
Download Length: 80 a.a. Molecular weight: 9071.48 Da Isoelectric Point: 4.5230
>AT103490 WP_000534858.1 NZ_CP028748:c2455849-2455610 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
Download Length: 240 bp
>AT103490 NZ_CP028748:c2455849-2455610 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 4FXE | |
| PDB | 2KC8 | |
| PDB | 2K29 | |
| AlphaFold DB | A0A4V1CTS8 |