Detailed information of TA system TA103488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2283163..2283801
• Replicon: chromosome
• Accession: NZ_CP028748
• Organism: Escherichia coli strain A27

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY80_RS11040
• Protein ID: WP_000813794.1
• Coordinates: 2283163..2283339 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY80_RS11045
• Protein ID: WP_001270286.1
• Coordinates: 2283385..2283801 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY80_RS11020 (2278782)	2278782..2279996	-	1215	WP_001326689.1	BenE family transporter YdcO	-
AOY80_RS11025 (2280049)	2280049..2280585	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AOY80_RS11030 (2280658)	2280658..2282619	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY80_RS11035 (2282711)	2282711..2282941	-	231	WP_000494244.1	YncJ family protein	-
AOY80_RS11040 (2283163)	2283163..2283339	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY80_RS11045 (2283385)	2283385..2283801	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY80_RS11050 (2283880)	2283880..2285286	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
AOY80_RS11055 (2285531)	2285531..2286676	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
AOY80_RS11060 (2286694)	2286694..2287707	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AOY80_RS11065 (2287708)	2287708..2288649	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vgrG/tssI / rhs/PAAR / rhs/PAAR / espR1	2092760..2505590	412830

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T103488 WP_000813794.1 NZ_CP028748:2283163-2283339 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103488 NZ_CP028748:2283163-2283339 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103488 WP_001270286.1 NZ_CP028748:2283385-2283801 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103488 NZ_CP028748:2283385-2283801 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB