Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1251915..1252533 Replicon chromosome
Accession NZ_CP028748
Organism Escherichia coli strain A27

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY80_RS06040 Protein ID WP_001291435.1
Coordinates 1251915..1252133 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY80_RS06045 Protein ID WP_000344800.1
Coordinates 1252159..1252533 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY80_RS06005 (1247204) 1247204..1247776 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY80_RS06010 (1247807) 1247807..1248118 - 312 WP_000409911.1 MGMT family protein -
AOY80_RS06020 (1248497) 1248497..1248850 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY80_RS06025 (1248892) 1248892..1250442 - 1551 WP_001408474.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY80_RS06030 (1250606) 1250606..1251076 - 471 WP_000136192.1 YlaC family protein -
AOY80_RS06035 (1251192) 1251192..1251743 - 552 WP_000102564.1 maltose O-acetyltransferase -
AOY80_RS06040 (1251915) 1251915..1252133 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY80_RS06045 (1252159) 1252159..1252533 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY80_RS06050 (1253079) 1253079..1256228 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY80_RS06055 (1256251) 1256251..1257444 - 1194 WP_086218294.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103487 WP_001291435.1 NZ_CP028748:c1252133-1251915 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103487 NZ_CP028748:c1252133-1251915 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103487 WP_000344800.1 NZ_CP028748:c1252533-1252159 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103487 NZ_CP028748:c1252533-1252159 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References