Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 1047828..1048507 | Replicon | chromosome |
| Accession | NZ_CP028748 | ||
| Organism | Escherichia coli strain A27 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | S1FHS4 |
| Locus tag | AOY80_RS05040 | Protein ID | WP_000854680.1 |
| Coordinates | 1047828..1048169 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | S1EWR7 |
| Locus tag | AOY80_RS05045 | Protein ID | WP_000070396.1 |
| Coordinates | 1048190..1048507 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AOY80_RS05015 (1042842) | 1042842..1043432 | + | 591 | WP_000980256.1 | host cell division inhibitor Icd-like protein | - |
| AOY80_RS05020 (1043425) | 1043425..1043712 | + | 288 | WP_001244670.1 | hypothetical protein | - |
| AOY80_RS05025 (1043705) | 1043705..1044160 | + | 456 | WP_021545977.1 | hypothetical protein | - |
| AOY80_RS05030 (1044296) | 1044296..1044616 | + | 321 | WP_000856729.1 | DUF5375 domain-containing protein | - |
| AOY80_RS05035 (1044631) | 1044631..1046964 | + | 2334 | WP_089591985.1 | primase-like DNA-binding domain-containing protein | - |
| AOY80_RS05040 (1047828) | 1047828..1048169 | - | 342 | WP_000854680.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| AOY80_RS05045 (1048190) | 1048190..1048507 | - | 318 | WP_000070396.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| AOY80_RS05050 (1048526) | 1048526..1048747 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| AOY80_RS05055 (1048756) | 1048756..1049232 | - | 477 | WP_000811693.1 | RadC family protein | - |
| AOY80_RS05060 (1049248) | 1049248..1049706 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| AOY80_RS05065 (1049804) | 1049804..1050043 | - | 240 | WP_000194654.1 | DUF905 family protein | - |
| AOY80_RS05070 (1050120) | 1050120..1050587 | - | 468 | WP_001385283.1 | protein YkfB | - |
| AOY80_RS05075 (1050610) | 1050610..1051053 | - | 444 | WP_000649865.1 | hypothetical protein | - |
| AOY80_RS05080 (1051053) | 1051053..1051289 | - | 237 | WP_001144031.1 | protein YpjK | - |
| AOY80_RS05085 (1051330) | 1051330..1052031 | - | 702 | WP_000189411.1 | WYL domain-containing protein | - |
| AOY80_RS05090 (1052248) | 1052248..1053071 | - | 824 | Protein_978 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12892.95 Da Isoelectric Point: 9.6543
>T103486 WP_000854680.1 NZ_CP028748:c1048169-1047828 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T103486 NZ_CP028748:c1048169-1047828 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11982.62 Da Isoelectric Point: 7.0270
>AT103486 WP_000070396.1 NZ_CP028748:c1048507-1048190 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT103486 NZ_CP028748:c1048507-1048190 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|