Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4499739..4500236 Replicon chromosome
Accession NZ_CP028747
Organism Escherichia coli strain A28

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY81_RS21965 Protein ID WP_001173343.1
Coordinates 4499739..4499912 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AOY81_RS21970 Protein ID WP_000398619.1
Coordinates 4499940..4500236 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY81_RS21955 (4496409) 4496409..4497866 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
AOY81_RS21960 (4498103) 4498103..4499620 + 1518 WP_001295074.1 lysine--tRNA ligase -
AOY81_RS21965 (4499739) 4499739..4499912 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY81_RS21970 (4499940) 4499940..4500236 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY81_RS21975 (4500463) 4500463..4500735 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY81_RS21980 (4500747) 4500747..4500977 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
AOY81_RS21985 (4501158) 4501158..4502789 + 1632 WP_001216461.1 sensor histidine kinase -
AOY81_RS21990 (4502786) 4502786..4503505 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103481 WP_001173343.1 NZ_CP028747:c4499912-4499739 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103481 NZ_CP028747:c4499912-4499739 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT103481 WP_000398619.1 NZ_CP028747:c4500236-4499940 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103481 NZ_CP028747:c4500236-4499940 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References