Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4438278..4438831 Replicon chromosome
Accession NZ_CP028747
Organism Escherichia coli strain A28

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag AOY81_RS21660 Protein ID WP_240760459.1
Coordinates 4438278..4438592 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID -
Locus tag AOY81_RS21665 Protein ID WP_240760460.1
Coordinates 4438595..4438831 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY81_RS21640 (4434048) 4434048..4434446 + 399 WP_240760455.1 cag pathogenicity island Cag12 family protein -
AOY81_RS21645 (4434482) 4434482..4434787 + 306 WP_240760456.1 conjugal transfer protein -
AOY81_RS21650 (4435907) 4435907..4437520 + 1614 WP_240760457.1 type IV secretion system DNA-binding domain-containing protein -
AOY81_RS21655 (4437529) 4437529..4438281 + 753 WP_240760458.1 MobC family replication-relaxation protein -
AOY81_RS21660 (4438278) 4438278..4438592 - 315 WP_240760459.1 CcdB family protein Toxin
AOY81_RS21665 (4438595) 4438595..4438831 - 237 WP_240760460.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY81_RS21670 (4439036) 4439036..4439236 + 201 Protein_4253 IS21 family transposase -
AOY81_RS21675 (4439251) 4439251..4439379 + 129 Protein_4254 ATP-binding protein -
AOY81_RS21680 (4439451) 4439451..4439555 - 105 Protein_4255 transposase -
AOY81_RS21685 (4439737) 4439737..4439877 - 141 Protein_4256 IS3 family transposase -
AOY81_RS21690 (4440576) 4440576..4441040 - 465 WP_240760461.1 transcriptional regulator -
AOY81_RS21695 (4441048) 4441048..4442232 - 1185 WP_240760462.1 aminotransferase class V-fold PLP-dependent enzyme -
AOY81_RS21700 (4442201) 4442201..4443361 - 1161 WP_240760463.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - afaC-I 4418351..4490269 71918


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11465.34 Da        Isoelectric Point: 8.8431

>T103480 WP_240760459.1 NZ_CP028747:c4438592-4438278 [Escherichia coli]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVVPLLSVEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASVPVRALG
TVYCDAAQYRSQVKAAIDFLIDGI

Download         Length: 315 bp

>T103480 NZ_CP028747:c4438592-4438278 [Escherichia coli]
ATGCAGTTCACCGTATACGGTAATACCGGTAAAAGCGCCGTTTACCCACTGTTGCTCGATGTCACGAGTGATATTATTGG
GCAACTGAACCGCCGGATAGTGGTCCCGTTGCTGTCTGTTGAAAAATATCCGGCAGGCCGCCGCCCGGATCGCCTTGTCC
CTGTTGTCAGACTGACGGACGGCAAGGAATACGCTGTAATGACTCACGAGCTGGCAAGTGTCCCTGTCCGGGCTCTGGGG
ACGGTGTACTGTGATGCTGCTCAGTATCGCTCTCAGGTAAAGGCCGCCATCGATTTCCTCATTGACGGCATTTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9072.09 Da        Isoelectric Point: 5.6350

>AT103480 WP_240760460.1 NZ_CP028747:c4438831-4438595 [Escherichia coli]
MTAKERNTQSVTMAVERTLLVRAREAGINLSATLTTALDAELRHYEAKKWQEENREAIEALNRFHDEHGCFSDEYRTF

Download         Length: 237 bp

>AT103480 NZ_CP028747:c4438831-4438595 [Escherichia coli]
ATGACAGCGAAAGAACGAAATACGCAGAGCGTGACAATGGCGGTGGAACGTACTCTGCTGGTCCGGGCGCGTGAGGCAGG
CATTAACCTGAGTGCGACCCTGACAACCGCCCTGGATGCGGAGCTTCGCCACTATGAAGCCAAAAAATGGCAGGAAGAGA
ACAGGGAGGCCATTGAGGCATTAAACCGTTTTCATGATGAACATGGCTGTTTCAGTGATGAATACAGGACATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References