Detailed information of TA system TA103478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3597511..3598348
• Replicon: chromosome
• Accession: NZ_CP028747
• Organism: Escherichia coli strain A28

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY81_RS17505
• Protein ID: WP_000227784.1
• Coordinates: 3597806..3598348 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY81_RS17500
• Protein ID: WP_001297137.1
• Coordinates: 3597511..3597822 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY81_RS17475 (3592531)	3592531..3593478	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY81_RS17480 (3593500)	3593500..3595491	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY81_RS17485 (3595481)	3595481..3596095	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY81_RS17490 (3596095)	3596095..3596424	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY81_RS17495 (3596436)	3596436..3597326	+	891	WP_000971336.1	heme o synthase	-
AOY81_RS17500 (3597511)	3597511..3597822	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY81_RS17505 (3597806)	3597806..3598348	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY81_RS17510 (3598404)	3598404..3599339	-	936	WP_001368479.1	tetratricopeptide repeat protein	-
AOY81_RS17515 (3599747)	3599747..3601111	+	1365	WP_001000960.1	MFS transporter	-
AOY81_RS17520 (3601239)	3601239..3601730	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY81_RS17525 (3601898)	3601898..3602809	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103478 WP_000227784.1 NZ_CP028747:3597806-3598348 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103478 NZ_CP028747:3597806-3598348 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103478 WP_001297137.1 NZ_CP028747:3597511-3597822 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103478 NZ_CP028747:3597511-3597822 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3