Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1461116..1461741 Replicon chromosome
Accession NZ_CP028747
Organism Escherichia coli strain A28

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag AOY81_RS07185 Protein ID WP_000911329.1
Coordinates 1461343..1461741 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag AOY81_RS07180 Protein ID WP_000450524.1
Coordinates 1461116..1461343 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY81_RS07155 (1456918) 1456918..1457388 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
AOY81_RS07160 (1457388) 1457388..1457960 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
AOY81_RS07165 (1458106) 1458106..1458984 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
AOY81_RS07170 (1459001) 1459001..1460035 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
AOY81_RS07175 (1460248) 1460248..1460961 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
AOY81_RS07180 (1461116) 1461116..1461343 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
AOY81_RS07185 (1461343) 1461343..1461741 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
AOY81_RS07190 (1461888) 1461888..1462751 + 864 WP_001267495.1 neutral zinc metallopeptidase -
AOY81_RS07195 (1462766) 1462766..1464781 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
AOY81_RS07200 (1464855) 1464855..1465553 + 699 WP_000679812.1 esterase -
AOY81_RS07205 (1465634) 1465634..1465834 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T103465 WP_000911329.1 NZ_CP028747:1461343-1461741 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T103465 NZ_CP028747:1461343-1461741 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT103465 WP_000450524.1 NZ_CP028747:1461116-1461343 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT103465 NZ_CP028747:1461116-1461343 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References