Detailed information of TA system TA103458

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4062226..4062944
• Replicon: chromosome
• Accession: NZ_CP028746
• Organism: Escherichia coli strain A29

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A1X0YHY7
• Locus tag: AOY82_RS19590
• Protein ID: WP_001585300.1
• Coordinates: 4062226..4062567 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AOY82_RS19595
• Protein ID: WP_001585299.1
• Coordinates: 4062588..4062944 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY82_RS19575 (4058179)	4058179..4059343	+	1165	Protein_3843	IS3-like element IS3 family transposase	-
AOY82_RS19580 (4060335)	4060335..4060508	-	174	Protein_3844	integrase	-
AOY82_RS19585 (4060960)	4060960..4061967	-	1008	WP_001585301.1	restriction endonuclease	-
AOY82_RS19590 (4062226)	4062226..4062567	-	342	WP_001585300.1	TA system toxin CbtA family protein	Toxin
AOY82_RS19595 (4062588)	4062588..4062944	-	357	WP_001585299.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY82_RS19600 (4062970)	4062970..4063191	-	222	WP_001585298.1	DUF987 domain-containing protein	-
AOY82_RS19605 (4063205)	4063205..4063651	-	447	WP_024195516.1	DNA repair protein RadC	-
AOY82_RS19610 (4063746)	4063746..4064216	-	471	WP_001585296.1	antirestriction protein	-
AOY82_RS19615 (4064295)	4064295..4065119	-	825	WP_201479570.1	DUF932 domain-containing protein	-
AOY82_RS19620 (4065206)	4065206..4066144	-	939	WP_201479562.1	hypothetical protein	-
AOY82_RS19625 (4066209)	4066209..4066619	-	411	WP_001585292.1	hypothetical protein	-
AOY82_RS19630 (4066735)	4066735..4066950	-	216	WP_001585291.1	hypothetical protein	-
AOY82_RS19635 (4066963)	4066963..4067112	-	150	WP_163396817.1	hypothetical protein	-
AOY82_RS19640 (4067114)	4067114..4067821	-	708	WP_001585289.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12932.96 Da
• Isoelectric Point: 8.9815

>T103458 WP_001585300.1 NZ_CP028746:c4062567-4062226 [Escherichia coli]
MKFLLATNLRSAKPCLSSVAVWQMLLERLLEKHYGLTLNDTPFSDETVIHEHIDAGISLADAVNLLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T103458 NZ_CP028746:c4062567-4062226 [Escherichia coli]
ATGAAATTTCTACTTGCAACAAACTTGCGGTCGGCGAAACCATGCCTGTCATCCGTTGCTGTCTGGCAAATGCTGCTTGA
ACGCCTGCTGGAAAAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCATGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATCTTCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGTAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACAGTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13284.01 Da
• Isoelectric Point: 6.4588

>AT103458 WP_001585299.1 NZ_CP028746:c4062944-4062588 [Escherichia coli]
MNNHSESGTTPENTSCPQWGLKRAITPCFGARLVQEGNRLHFLPDRAGFNCAFSDDDSLRLDQAFPLILKQLELMLTSRE
LSPRYQHCVTLYHNGLTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 357 bp

>AT103458 NZ_CP028746:c4062944-4062588 [Escherichia coli]
ATGAACAATCACTCCGAATCCGGTACAACGCCGGAGAATACCTCCTGCCCGCAGTGGGGGCTCAAACGTGCTATTACACC
TTGTTTTGGTGCCCGCCTGGTACAGGAGGGAAACCGACTGCATTTTCTCCCTGACCGGGCCGGATTTAACTGTGCTTTCA
GCGACGATGACTCGCTGCGCCTGGATCAGGCCTTTCCGTTGATACTCAAGCAGCTGGAGCTGATGCTCACCAGCAGAGAA
CTATCTCCCCGGTATCAGCACTGCGTCACGCTATATCACAACGGGCTCACTTGCGAAGCCGACACACTTGGCAGTTGCGG
TTACGTATACATCGCCGTTTATCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A1X0YHY7