Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3458139..3458757 Replicon chromosome
Accession NZ_CP028746
Organism Escherichia coli strain A29

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY82_RS16710 Protein ID WP_001291435.1
Coordinates 3458539..3458757 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY82_RS16705 Protein ID WP_000344800.1
Coordinates 3458139..3458513 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY82_RS16695 (3453228) 3453228..3454421 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY82_RS16700 (3454444) 3454444..3457593 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY82_RS16705 (3458139) 3458139..3458513 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY82_RS16710 (3458539) 3458539..3458757 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY82_RS16715 (3458929) 3458929..3459480 + 552 WP_000102564.1 maltose O-acetyltransferase -
AOY82_RS16720 (3459596) 3459596..3460066 + 471 WP_000136192.1 YlaC family protein -
AOY82_RS16725 (3460230) 3460230..3461780 + 1551 WP_053275205.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY82_RS16730 (3461822) 3461822..3462175 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY82_RS16740 (3462554) 3462554..3462865 + 312 WP_000409911.1 MGMT family protein -
AOY82_RS16745 (3462896) 3462896..3463468 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103456 WP_001291435.1 NZ_CP028746:3458539-3458757 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103456 NZ_CP028746:3458539-3458757 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103456 WP_000344800.1 NZ_CP028746:3458139-3458513 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103456 NZ_CP028746:3458139-3458513 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References