Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2485391..2486029 Replicon chromosome
Accession NZ_CP028746
Organism Escherichia coli strain A29

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AOY82_RS12000 Protein ID WP_000813794.1
Coordinates 2485853..2486029 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AOY82_RS11995 Protein ID WP_001270286.1
Coordinates 2485391..2485807 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY82_RS11975 (2480543) 2480543..2481484 - 942 WP_001251313.1 ABC transporter permease -
AOY82_RS11980 (2481485) 2481485..2482498 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
AOY82_RS11985 (2482516) 2482516..2483661 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
AOY82_RS11990 (2483906) 2483906..2485312 - 1407 WP_053275309.1 PLP-dependent aminotransferase family protein -
AOY82_RS11995 (2485391) 2485391..2485807 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AOY82_RS12000 (2485853) 2485853..2486029 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AOY82_RS12005 (2486251) 2486251..2486481 + 231 WP_000494244.1 YncJ family protein -
AOY82_RS12010 (2486573) 2486573..2488534 - 1962 WP_053275310.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AOY82_RS12015 (2488607) 2488607..2489143 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AOY82_RS12020 (2489235) 2489235..2490410 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T103455 WP_000813794.1 NZ_CP028746:c2486029-2485853 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T103455 NZ_CP028746:c2486029-2485853 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT103455 WP_001270286.1 NZ_CP028746:c2485807-2485391 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT103455 NZ_CP028746:c2485807-2485391 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References