Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 320857..321488 Replicon chromosome
Accession NZ_CP028746
Organism Escherichia coli strain A29

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag AOY82_RS01460 Protein ID WP_001259388.1
Coordinates 320857..321132 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY82_RS01465 Protein ID WP_000593555.1
Coordinates 321129..321488 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY82_RS01445 (315859) 315859..316926 + 1068 WP_000361470.1 HlyD family secretion protein -
AOY82_RS01450 (316923) 316923..319660 + 2738 Protein_287 ribosome-associated ATPase/putative transporter RbbA -
AOY82_RS01455 (319660) 319660..320784 + 1125 WP_001216257.1 ABC transporter permease -
AOY82_RS01460 (320857) 320857..321132 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY82_RS01465 (321129) 321129..321488 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY82_RS01470 (321608) 321608..322009 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOY82_RS01475 (322015) 322015..322821 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
AOY82_RS01480 (322818) 322818..323582 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
AOY82_RS01485 (323582) 323582..324415 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AOY82_RS01490 (324412) 324412..325356 - 945 WP_000947062.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 313238..321488 8250


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T103440 WP_001259388.1 NZ_CP028746:320857-321132 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103440 NZ_CP028746:320857-321132 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103440 WP_000593555.1 NZ_CP028746:321129-321488 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103440 NZ_CP028746:321129-321488 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References